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Home >> Antibodies >> Recombinant NF-H Antibody / Neurofilament Heavy

Recombinant NF-H Antibody / Neurofilament Heavy [clone NFHP-1R] (V7413)

  Catalog No Formulation Size Price (USD)  
Image V7413-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7413-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7413SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7413IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human brain lysate with recombinant NF-H antibody (clone NFHP-1R). Expected molecular weight ~200 kDa.
IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NFHP-1R
Purity Protein A affinity
UniProt P12036
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant NF-H antibody is available for research use only.
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Description

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.

Storage

Store the recombinant NF-H antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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