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Home >> Antibodies >> Recombinant Neurofilament Antibody / Heavy

Recombinant Neurofilament Antibody / Heavy [clone rNF421] (V3643)

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  Catalog No Formulation Size Price (USD)  
Image V3643-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3643-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3643SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3643IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human brain with recombinant Neurofilament antibody (clone rNF421). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human brain with recombinant Neurofilament antibody (clone rNF421). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Neurofilament antibody (clone rNF421) as confirmation of integrity and purity.
Flow cytometry testing of permeabilized human HEK293 cells with recombinant Neurofilament antibody (clone rNF421); Red=isotype control, Blue= recombinant Neurofilament antibody.
Western blot testing of human brain lysate with recombinant Neurofilament antibody (clone rNF421).
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rNF421
Purity Protein G affinity
UniProt P12036
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Flow cytometry : 0.5-1ug/10^6 cells
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Neurofilament antibody is available for research use only.
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Description

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Application Notes

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the recombinant Neurofilament antibody to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

Storage

Store the recombinant Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all Neurofilament antibodies

   2.   View all recombinant antibodies

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