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Home >> Antibodies >> Recombinant Neurofilament Antibody (light chain)

Recombinant Neurofilament Antibody (light chain) [clone RM280] (R20297)

  Catalog No Formulation Size Price (USD)  
Image R20297-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human brain lysate using recombinant Neurofilament antibody at 1:2500. Predicted molecular weight: 62-68 kDa.
IHC testing of FFPE human brain tissue with recombinant Neurofilament antibody at 1:2500.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM280
Purity Protein A purified from animal origin-free supernatant
UniProt P07196
Gene ID 4747
Applications Immunohistochemistry (FFPE) : 1:1000-1:2500 (1)
Western blot : 1:1000-1:2500
Limitations This recombinant Neurofilament antibody is available for research use only.
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Description

This recombinant Neurofilament antibody (light chain) reacts to human Neurofilament-L (NF-L).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Neurofilament antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Neurofilament-L (NF-L) was used as the immunogen for this recombinant Neurofilament antibody.

Storage

Store the recombinant Neurofilament antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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