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Home >> Antibodies >> Recombinant NeuN Antibody / Fox3

Recombinant NeuN Antibody / Fox3 [clone NeuN/6694R] (V9609)

  Catalog No Formulation Size Price (USD)  
Image V9609-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9609-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9609SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human brain tissue with recombinant NeuN antibody (clone NeuN/6694R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.
IHC staining of FFPE human ganglioma tissue with recombinant NeuN antibody (clone NeuN/6694R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebrum tissue with recombinant NeuN antibody (clone NeuN/6694R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NeuN/6694R
Purity Protein A/G affinity
UniProt A6NFN3
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant NeuN antibody is available for research use only.
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Description

NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Application Notes

Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.

Immunogen

A portion of amino acids aa30-60 from the human protein was used as the immunogen for the recombinant NeuN antibody.

Storage

Aliquot the recombinant NeuN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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