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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant NeuN Antibody / Fox3 / N-Terminal

Recombinant NeuN Antibody / Fox3 / N-Terminal [clone RM312] (R20331)

  Catalog No Formulation Size Price (USD)  
R20331-0.1ML Antibody in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide 100 ul 369
Western blot testing of human brain lysate with recombinant NeuN antibody at 1:1000 dilution. Western blot testing of human fetal brain lysate with NeuN antibody at 0.5ug/ml. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant NeuN antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM312
Purity Protein A purified from animal origin-free supernatant
UniProt A6NFN3
Localization Nuclear, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1000
Western Blot : 1:1000-1:2000
Limitations This recombinant NeuN antibody is available for research use only.
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Description

RNA binding protein fox-1 homolog 3 (RBFOX3), also called Neuronal nuclei antigen and Fox-1 homolog C, is a pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant NeuN antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the N-terminus of human NeuN/RBFOX3 was used as the immunogen for the recombinant NeuN antibody.

Storage

Store the recombinant NeuN antibody at -20oC.

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