NeuN Antibody / RBFOX3 N-Terminal Epitope Antibody [clone RM312] (R20331)

NeuN Antibody N-Terminal WB. Western blot analysis of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in human brain lysate using recombinant rabbit monoclonal NeuN antibody, clone RM312, at 1:1000 dilution. A band is detected at approximately 46-48 kDa, consistent with the expected molecular weight of RBFOX3 isoforms, supporting detection of this neuronal nuclear protein with an N-terminal epitope-targeting antibody.

NeuN Antibody N-Terminal Brain IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in formalin-fixed, paraffin-embedded human brain tissue using recombinant rabbit monoclonal NeuN antibody, clone RM312, at 1:1000 dilution. HRP-DAB brown staining highlights nuclear labeling of neurons with scattered cytoplasmic signal, consistent with NeuN expression in mature neuronal populations, while surrounding non-neuronal cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling prior to staining.

NeuN Antibody N-Terminal Neuronal IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in formalin-fixed, paraffin-embedded human brain tissue using recombinant rabbit monoclonal NeuN antibody, clone RM312, at 1:1000 dilution. HRP-DAB brown staining highlights strong nuclear labeling of neurons with clear visualization of neuronal cell bodies and processes, consistent with NeuN expression in mature neuronal populations, while surrounding non-neuronal cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling prior to staining.

NeuN Antibody N-Terminal Mouse Brain IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in formalin-fixed, paraffin-embedded mouse brain tissue using recombinant rabbit monoclonal NeuN antibody, clone RM312, at 1:1000 dilution. HRP-DAB brown staining highlights widespread nuclear labeling of neurons throughout the brain parenchyma, consistent with NeuN expression in mature neuronal populations, while surrounding non-neuronal cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling prior to staining.