NAPSA Antibody / Protein Processing Antibody [clone NAPSA/4400R] (V8740)

NAPSA Antibody for IHC. Immunohistochemistry analysis of Napsin A (NAPSA) expression in FFPE human lung adenocarcinoma demonstrates strong granular cytoplasmic HRP-DAB brown staining in tumor epithelial cells with minimal background in surrounding stromal regions. Clone NAPSA/4400R highlights Napsin A expression consistent with its role in proteolytic maturation and epithelial protein processing, supporting its use as a protein processing marker in pulmonary tissues. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

IHC staining of FFPE human lung adenocarcinoma with recombinant Napsin A antibody (clone NAPSA/4400R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Napsin A antibody (clone NAPSA/4400R). These results demonstrate the foremost specificity of the NAPSA/4400R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

NAPSA Antibody for WB. Western blot analysis of Napsin A (NAPSA) expression in human kidney tissue lysate demonstrates a band at approximately 38-45 kDa, consistent with the predicted molecular weight of this glycosylated lysosomal aspartic protease. Clone NAPSA/4400R detects Napsin A with a defined band corresponding to processed protein species, reflecting proteolytic maturation associated with its role in epithelial protein processing.