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Home >> Antibodies >> Recombinant MYOG Antibody / Myogenin / Myf-4

Recombinant MYOG Antibody / Myogenin / Myf-4 [clone rMYOG/6297] (V9676)

  Catalog No Formulation Size Price (USD)  
Image V9676-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9676-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9676SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human rhabdomyosarcoma tissue with recombinant MYOG antibody (clone rMYOG/6297). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant MYOG antibody (clone rMYOG/6297) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rMYOG/6297
Purity Protein A/G affinity
UniProt P15173
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant MYOG antibody is available for research use only.
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Description

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Application Notes

Optimal dilution of the recombinant MYOG antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant MYOG antibody.

Storage

Aliquot the recombinant MYOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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