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Home >> Antibodies >> Recombinant Myeloperoxidase Antibody / MPO

Recombinant Myeloperoxidase Antibody / MPO [clone MPO/33R] (V9350)

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  Catalog No Formulation Size Price (USD)  
Image V9350-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9350-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9350SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human spleen tissue with recombinant Myeloperoxidase antibody (clone MPO/33R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human spleen tissue with recombinant Myeloperoxidase antibody (clone MPO/33R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant Myeloperoxidase antibody (clone MPO/33R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human spleen tissue lysate using recombinant Myeloperoxidase antibody (clone MPO/33R). Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MPO/33R
Purity Protein A/G affinity
UniProt P05164
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Myeloperoxidase antibody is available for research use only.
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Description

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

Application Notes

Optimal dilution of the recombinant Myeloperoxidase antibody should be determined by the researcher.

Immunogen

A portion of amino acids 150-250 was used as the immunogen for the recombinant Myeloperoxidase antibody.

Storage

Aliquot the recombinant Myeloperoxidase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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