- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Myelin Basic Protein Antibody / CNS Myelin Marker [clone MBP/4277R] (V8436)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V8436-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V8436-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V8436SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | MBP/4277R |
| Purity | Protein A affinity chromatography |
| UniProt | P02686 |
| Localization | Cell surface, cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This Myelin Basic Protein Antibody / CNS Myelin Marker is available for research use only. |
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Myelin Basic Protein Antibody / CNS Myelin Marker detects myelin basic protein (MBP), a major structural component of the myelin sheath encoded by the MBP gene. MBP is highly expressed by oligodendrocytes in the central nervous system (CNS), where it plays an important role in the formation, compaction and stability of myelin. By supporting the specialized membrane structure that surrounds axons, MBP contributes to efficient nerve impulse conduction and normal nervous system function.
MBP is a highly basic protein concentrated primarily on the cytoplasmic face of compact myelin membranes. Its interactions with membrane lipids help bring adjacent membrane layers together and maintain the tightly packed organization of the myelin sheath. Alternative splicing of the MBP gene produces multiple protein isoforms that vary in molecular weight and show developmental differences in expression. These properties have made MBP an established CNS myelin marker for investigating oligodendrocyte differentiation, myelin formation and maturation.
Changes in MBP expression, localization and integrity are studied in conditions involving myelin loss or damage. MBP is particularly relevant to multiple sclerosis and experimental autoimmune encephalomyelitis, where it can function as a myelin-associated autoantigen. MBP expression is also examined in studies of demyelination and remyelination, traumatic injury, neurodegeneration and nervous system development. Detection of this CNS myelin marker can therefore provide information about myelin distribution and changes associated with oligodendrocyte and white matter biology.
Clone MBP/4277R is a recombinant rabbit monoclonal antibody validated for MBP detection by immunohistochemistry and Western blot using human brain samples. Recombinant production provides a defined antibody sequence and supports manufacturing consistency. The antibody has also been evaluated on a HuProt protein microarray containing more than 19,000 full-length human proteins. MBP produced the dominant signal with a Z-score of 42.24 and an S-score of 22.43. The Z-score reflects signal intensity relative to proteins across the array, while the S-score reflects separation of the intended target from other detected proteins, providing additional evidence of target specificity.
NSJ Bioreagents supplies antibodies for research into neural proteins, glial cells and nervous system biology. A Myelin Basic Protein Antibody can be used to investigate MBP expression and distribution in studies of CNS myelin, oligodendrocyte biology, myelin development and mechanisms associated with demyelination and remyelination.
For related reagents used to investigate myelin biology, glial cells and nervous system function, explore our Neuroscience Antibodies page.
Optimal dilution of the Myelin Basic Protein Antibody / CNS Myelin Marker should be determined by the researcher.
A portion of amino acids 150-250 from the human protein was used as the immunogen for the recombinant Myelin Basic Protein antibody.
Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
MBP antibody, Myelin Basic Protein antibody, CNS Myelin Marker antibody
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![Myelin Basic Protein Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in rat brain tissue.](https://www.nsjbio.com/plp/thumb_image/img-RQ5003-1.jpg?v=1789042989 "Myelin Basic Protein Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in rat brain tissue.")
