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Home >> Antibodies >> Recombinant MUC2 Antibody

Recombinant MUC2 Antibody [clone MLP/2970R] (V7308)

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  Catalog No Formulation Size Price (USD)  
Image V7308-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7308-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7308SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7308IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon tissue with recombinant MUC2 antibody (clone MLP/2970R). HIER: boil tissue sections in 10mM Tris-HCl buffer, pH 10, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with recombinant MUC2 antibody (clone MLP/2970R). HIER: boil tissue sections in 10mM Tris-HCl buffer, pH 10, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant MUC2 antibody (clone MLP/2970R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MLP/2970R
Purity Protein A affinity chromatography
UniProt Q02817
Localization Cytoplasmic and cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Limitations This recombinant MUC2 antibody is available for research use only.
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Description

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Application Notes

Titering of the recombinant MUC2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant MUC2 antibody. Its epitope has been defined as GTQTP.

Storage

Store the recombinant MUC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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