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Home >> Antibodies >> Recombinant MUC1 Antibody / Mucin-1

Recombinant MUC1 Antibody / Mucin-1 [clone MUC1/2729R] (V7307)

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  Catalog No Formulation Size Price (USD)  
Image V7307-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7307-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7307SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7307IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human prostate carcinoma with recombinant MUC1 antibody (clone MUC1/2729R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human prostate carcinoma with recombinant MUC1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant MUC1 antibody as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MUC1/2729R
Purity Protein A affinity chromatography
UniProt P15941
Gene ID 4582
Localization Cytoplasmic and cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant MUC1 antibody is available for research use only.
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Description

This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA/Mucin-1/MUC1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to MUC1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Application Notes

Titration of the recombinant MUC1 may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length human protein was used as the immunogen for this recombinant MUC1 antibody.

Storage

Store the recombinant MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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