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Home >> Antibodies >> Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase

Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase [clone SMTP-1R] (V7321)

  Catalog No Formulation Size Price (USD)  
Image V7321-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7321-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7321SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7321IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
Microvalidated Recrabbitmono
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Western blot testing of human HeLa cell lysate with recombinant MTAP antibody (clone SMTP-1R). Expected molecular weight: 26-38 kDa (multiple isoforms).
IHC staining of FFPE human kidney with recombinant MTAP antibody (clone SMTP-1R). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SMTP-1R
Purity Protein A affinity chromatography
UniProt Q13126
Localization Cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC Only Format : incubate for 30 min at RT (1)
Limitations This recombinant MTAP antibody is available for research use only.
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Description

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]

For knockdown-validated detection of MTAP as a metabolic marker, see our MTAP antibody.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant MTAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

Storage

Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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