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Home >> Antibodies >> Recombinant MSH6 Antibody

Recombinant MSH6 Antibody [clone MSH6/6654R] (V9182)

  Catalog No Formulation Size Price (USD)  
Image V9182-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9182-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9182SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue from a patient with Lynch syndrome using recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human HCT-116 cell lysate using recombinant MSH6 antibody (clone MSH6/6654R). Expected molecular weight: 120-160 kDa depending on phosphorylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name MSH6/6654R
Purity Protein A/G affinity
UniProt P52701
Localization Nuclear
Applications ELISA (order BSA-free format for coating) :
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant MSH6 antibody is available for research use only.
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Description

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H).

Application Notes

Optimal dilution of the recombinant MSH6 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-100 was used as the immunogen for the recombinant MSH6 antibody.

Storage

Aliquot the recombinant MSH6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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