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Home >> Antibodies >> Recombinant MSH2 Antibody

Recombinant MSH2 Antibody [clone rMSH2/6548] (V9291)

  Catalog No Formulation Size Price (USD)  
Image V9291-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9291-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9291SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cat lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE dog lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HCT-116 cell lysate using recombinant MSH2 antibody (clone rMSH2/6548). Expected molecular weight ~105 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant MSH2 antibody (clone rMSH2/6548) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Cat, Dog
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rMSH2/6548
Purity Protein A/G affinity
UniProt IDP43246
Localization Nucleus
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant MSH2 antibody is available for research use only.
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Description

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Application Notes

Optimal dilution of the recombinant MSH2 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human MSH2 protein was used as the immunogen for the recombinant MSH2 antibody.

Storage

Aliquot the recombinant MSH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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