Myeloperoxidase Antibody / Neutrophil Marker Antibody [clone rMPO/6904] (V9329)

Myeloperoxidase (MPO) Antibody Human Bone Marrow IHC. Immunohistochemistry staining of FFPE human bone marrow tissue using Myeloperoxidase Antibody / Neutrophil Marker Antibody (clone rMPO/6904) demonstrates strong granular cytoplasmic HRP-DAB brown staining in numerous granulocytic cells and developing myeloid precursors throughout the marrow compartment. The staining pattern is consistent with the established localization of Myeloperoxidase (MPO), a heme-containing peroxidase enzyme abundantly expressed within neutrophils and cells undergoing granulocytic differentiation. Non-granulocytic hematopoietic populations exhibit minimal staining, highlighting the lineage specificity of MPO expression within the myeloid compartment. The robust cytoplasmic immunoreactivity observed in maturing granulocytes is consistent with the widespread use of MPO as a marker of neutrophilic differentiation, myeloid lineage identification, and hematopoietic cell characterization. HIER: boil tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 20 minutes and allow to cool before staining.

Myeloperoxidase (MPO) Antibody Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil tissue using Myeloperoxidase Antibody / Neutrophil Marker Antibody (clone rMPO/6904) demonstrates distinct granular cytoplasmic HRP-DAB brown staining within scattered myeloid and granulocytic cells localized predominantly within interfollicular and stromal regions. The staining pattern is consistent with expression of Myeloperoxidase (MPO), a heme-containing peroxidase enzyme characteristically expressed by neutrophils, granulocytes, and other cells of myeloid lineage. MPO-positive cells are readily distinguished from the surrounding lymphoid populations, which show little to no staining, highlighting the specificity of MPO as a marker of granulocytic differentiation and innate immune cell identification. HIER: boil tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 20 minutes and allow to cool before testing.

Myeloperoxidase (MPO) Antibody Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue using Myeloperoxidase Antibody / Neutrophil Marker Antibody (clone rMPO/6904) demonstrates strong granular cytoplasmic HRP-DAB brown staining in numerous myeloid lineage cells distributed throughout the splenic tissue. The staining pattern is consistent with expression of Myeloperoxidase (MPO), a heme-containing peroxidase enzyme abundantly expressed in neutrophils, granulocytes, and developing myeloid cells. MPO-positive cells are readily identifiable against a background of largely negative lymphoid populations, highlighting the specificity of MPO as a marker of granulocytic differentiation and innate immune cell identification. The dense distribution of positively stained cells is consistent with the presence of myeloid and inflammatory cell populations within the spleen. HIER: boil tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 20 minutes and allow to cool before testing.

Myeloperoxidase (MPO) Antibody Human Spleen WB. Western blot analysis of human spleen tissue lysate using Myeloperoxidase Antibody / Neutrophil Marker Antibody (clone rMPO/6904) demonstrates a prominent immunoreactive band at approximately 60-70 kDa, consistent with the expected molecular weight of the mature Myeloperoxidase (MPO) heavy chain. MPO is a heme-containing lysosomal peroxidase abundantly expressed in neutrophils, granulocytes, and other myeloid lineage cells, where it contributes to antimicrobial defense through the generation of reactive oxidant species. The observed molecular weight is consistent with the heavily glycosylated nature of MPO, which frequently migrates above its calculated molecular mass during SDS-PAGE analysis. Detection in spleen tissue is consistent with the presence of resident and circulating myeloid cell populations expressing MPO. Expected molecular weight: approximately 59-64 kDa for the mature heavy chain, with higher molecular weight glycosylated and precursor forms potentially observed in myeloid tissues. Observed molecular weight: ~60-70 kDa.

SDS-PAGE analysis of purified, BSA-free MPO / Myeloperoxidase antibody (clone rMPO/6904) as confirmation of integrity and purity.