Mouse Lambda Light Chain Antibody RM110 / Mouse Lambda Antibody [clone RM110] (R20161)

Mouse Lambda Light Chain Antibody RM110 Specificity for Mouse IgG1 Lambda WB. Western blot testing of non-reduced and reduced mouse IgG1 kappa and IgG1 lambda with Mouse Lambda Light Chain Antibody clone RM110 at 0.2 ug/ml demonstrates a strong signal for non-reduced IgG1 lambda, while the corresponding kappa sample and reduced samples show no detectable signal. Each lane contained 20 ng of immunoglobulin. The selective recognition under non-reducing conditions demonstrates the specificity of the Mouse Lambda Antibody for lambda-containing mouse immunoglobulin under the tested conditions.

Mouse Lambda Light Chain Antibody RM110 Immunoglobulin Specificity ELISA. ELISA testing with Mouse Lambda Light Chain Antibody clone RM110 demonstrates concentration-dependent recognition of mouse lambda-containing immunoglobulins at 200, 50 and 10 ng/ml. Little to no corresponding signal is observed with mouse kappa light chain or the tested human, rat and goat IgG samples. The results demonstrate selective recognition of mouse lambda light chain by the Mouse Lambda Antibody across the tested antibody concentrations.

Mouse Lambda Light Chain Antibody RM110 Titration ELISA. ELISA titration of Mouse Lambda Light Chain Antibody clone RM110 against decreasing amounts of immobilized mouse IgG1 lambda demonstrates dose-dependent binding across serial antibody dilutions. Higher coating amounts produce stronger absorbance signals, while progressively lower antigen concentrations yield correspondingly reduced responses. The titration demonstrates concentration-dependent recognition of mouse lambda-containing immunoglobulin by the Mouse Lambda Antibody.