Mouse Kappa Light Chain Antibody for ELISA RM103 / Rabbit Monoclonal [clone RM103] (R20162)

Mouse Kappa Light Chain Antibody RM103 Specificity WB. Western blot testing of non-reduced (-) and reduced (+) mouse IgG1 kappa and IgG1 lambda with Mouse Kappa Light Chain Antibody clone RM103 demonstrates strong recognition of non-reduced IgG1 kappa at approximately 150 kDa and weaker recognition of the reduced kappa light chain near 25 kDa. No corresponding bands are visible in the IgG1 lambda lanes. The results demonstrate selective recognition of mouse kappa light chain under the tested conditions.

Mouse Kappa Light Chain Antibody RM103 Species Specificity ELISA. ELISA testing of Mouse Kappa Light Chain Antibody clone RM103 against a panel of immunoglobulins demonstrates strong recognition of mouse kappa-containing immunoglobulins at 200 ng/ml, with progressively lower signals at 50 and 10 ng/ml. Mouse lambda and the tested human, rat, and goat IgG samples show minimal reactivity by comparison, demonstrating preferential detection of mouse kappa light chain.

Mouse Kappa Light Chain Antibody RM103 Titration ELISA. ELISA titration of Mouse Kappa Light Chain Antibody clone RM103 against immobilized mouse IgG1 kappa demonstrates concentration-dependent detection across serial antibody dilutions. Higher concentrations of RM103 produce stronger signals, with the response progressively decreasing as the antibody is diluted and converging toward background at the lowest concentrations. The titration demonstrates dose-responsive recognition of mouse kappa light chain across the tested ELISA conditions.