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Home >> Antibodies >> Recombinant Mouse Kappa Light Chain Antibody

Recombinant Mouse Kappa Light Chain Antibody [clone RM103] (R20162)

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  Catalog No Formulation Size Price (USD)  
Image R20162-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
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Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mIgs. No cross reactivity with the lambda light chain or human/rat/goat IgG (κ+λ).
ELISA titer: the plate was coated with different amounts of mouse IgG1κ. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM103
Purity Protein A purified from animal origin-free supernatant
UniProt P01837
Gene ID 16071
Applications ELISA : 0.005-0.2ug/ml
Western Blot (non-reduced) : 0.1-0.5ug/ml
Limitations This recombinant Mouse Kappa Light Chain antibody is available for research use only.
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Description

This recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of murine immunoglobulins. No cross reactivity with the lamda chain or human/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse Kappa Light Chain antibody.

Storage

Store the recombinant Mouse Kappa Light Chain antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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