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Home >> Antibodies >> Recombinant Mouse Kappa Light Chain Antibody (Biotin Conjugate)

Recombinant Mouse Kappa Light Chain Antibody (Biotin Conjugate) [clone RM103] (R20162BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20162BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ). The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ).
Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/ml of recombinant Mouse Kappa Light Chain antibody. This mAb reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of recombinant Mouse Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM103
Purity Protein A purified from animal origin-free supernatant
UniProt P01837
Gene ID 16071
Applications ELISA : 0.005-0.2ug/ml
Western Blot (non-reduced) : 0.1-0.5ug/ml
Limitations This recombinant Mouse Kappa Light Chain antibody is available for research use only.
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Description

This recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse Kappa Light Chain antibody.

Storage

Store the recombinant Mouse Kappa Light Chain antibody at -20oC.

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