Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate / Biotin Anti-Mouse Kappa Antibody [clone RM103] (R20162BTN)

Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate Specificity ELISA. ELISA testing of Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate against different immunoglobulins demonstrates strong recognition of mouse kappa-containing immunoglobulins at 200 ng/ml, with progressively lower signals at 50 and 10 ng/ml. Mouse lambda and the tested human, rat, and goat immunoglobulins show minimal reactivity by comparison. The results demonstrate preferential recognition of mouse kappa light chain by this Biotin Anti-Mouse Kappa Antibody.

Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate Specificity WB. Western blot testing of non-reduced (-) and reduced (+) mouse IgG1 kappa and IgG1 lambda with Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate demonstrates strong recognition of non-reduced IgG1 kappa at approximately 150-160 kDa and weaker recognition of the reduced kappa light chain near 25 kDa. No corresponding bands are visible in the IgG1 lambda lanes. The results demonstrate selective recognition of mouse kappa light chain by this Biotin Anti-Mouse Kappa Antibody under the tested conditions.

Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate Titration ELISA. ELISA titration of Mouse Kappa Light Chain Antibody RM103 Biotin Conjugate against increasing amounts of immobilized mouse IgG1 kappa demonstrates concentration-dependent detection across serial antibody dilutions. Higher concentrations of RM103 produce stronger signals, with responses progressively decreasing as the antibody is diluted and approaching background at the lowest concentrations. This Biotin Anti-Mouse Kappa Antibody demonstrates dose-responsive recognition of mouse kappa light chain across the tested ELISA conditions.