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Home >> Antibodies >> Recombinant Mouse IgG3 Antibody

Recombinant Mouse IgG3 Antibody [clone RM218] (R20168)

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  Catalog No Formulation Size Price (USD)  
Image R20168-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG3 antibody reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG3 antibody reacts to both IgG3κ and IgG3λ; No cross reactivity with IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
ELISA titration: he plate was coated with different amounts of mouse IgG3. A serial dilution of recombinant Mouse IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM218
Purity Protein A purified from animal origin-free supernatant
UniProt P03987
Gene ID 380795
Applications ELISA : 0.005-0.2ug/ml
Limitations This recombinant Mouse IgG3 antibody is available for research use only.
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Description

This recombinant Mouse IgG3 antibody reacts to the Fab region of mIgG3. No cross reactivity with IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG3 was used as the immunogen for this recombinant Mouse IgG3 antibody.

Storage

Store the recombinant Mouse IgG3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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