- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Mouse IgG3 Antibody for ELISA / Biotinylated Anti-Mouse IgG3 ELISA Detection Antibody [clone RM218] (R20168BTN)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20168BTN-50UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 50 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse |
| Format | Biotin Conjugate |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM218 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P03987 |
| Gene ID | 380795 |
| Applications | ELISA : 0.005-0.2ug/ml |
| Limitations | This Mouse IgG3 Antibody for ELISA / Biotinylated Anti-Mouse IgG3 ELISA Detection Antibody is available for research use only. |
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Mouse immunoglobulin gamma 3 (IgG3) is a unique IgG subclass in murine systems defined by its extended hinge region and strong propensity for immune complex formation. IgG3 is predominantly produced in response to T-independent antigens, particularly polysaccharides, and serves as an early indicator of humoral immune activation. Its structural characteristics promote multimerization and antigen cross-linking, distinguishing it functionally from other IgG subclasses such as IgG1, IgG2a, and IgG2b.
Mouse IgG3 Antibody for ELISA / Biotinylated Anti-Mouse IgG3 ELISA Detection Antibody is engineered for enhanced sensitivity and subclass-specific detection in ELISA workflows utilizing streptavidin-based signal amplification systems. Mouse IgG3 antibody, also referred to as anti-IgG3 antibody or anti-mouse IgG3 subclass antibody, is widely used for detecting and quantifying IgG3 with high specificity. This biotinylated recombinant rabbit monoclonal antibody clone RM218 selectively recognizes the Fab region of mouse IgG3, enabling precise detection of the antigen-binding domain and providing a complementary approach to Fc-directed assays.
In sandwich ELISA configurations, biotinylated detection antibodies provide increased assay sensitivity and expanded dynamic range through streptavidin-mediated amplification. The Mouse IgG3 Antibody for ELISA / Biotinylated Anti-Mouse IgG3 ELISA Detection Antibody binds selectively to captured IgG3, allowing accurate detection even at low analyte concentrations. Fab-directed recognition further enhances assay specificity by reducing Fc-associated background interactions, making this antibody particularly useful in complex biological samples.
Clone RM218 antibody targets the Fab region of mouse IgG3, ensuring selective detection without cross-reactivity to other mouse IgG subclasses or immunoglobulin classes. The recombinant rabbit monoclonal format provides strong affinity, consistent performance, and reproducibility across ELISA platforms. Biotin conjugation enhances assay flexibility by enabling compatibility with streptavidin-based detection systems commonly used in ELISA assays.
Measurement of IgG3 using biotinylated detection antibodies is widely applied in immunology research, antibody development, and studies of T-independent immune responses. Because IgG3 plays a distinct role in immune complex formation and early immune activation, accurate detection provides insight into antibody response mechanisms and functional immune dynamics. This antibody supports these applications by enabling sensitive and selective detection of IgG3 immunoglobulins in ELISA-based systems requiring enhanced signal amplification, high specificity, and consistent assay performance.
This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.
The stated application concentrations are suggested starting points. Titration of the Mouse IgG3 Antibody for ELISA / Biotinylated Anti-Mouse IgG3 ELISA Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Mouse IgG3 was used as the immunogen for this biotinylated recombinant Mouse IgG3 antibody.
Store the recombinant Mouse IgG3 antibody at -20oC.
Biotin anti-mouse IgG3 antibody, Biotinylated IgG3 detection antibody, Mouse IgG3 biotin ELISA antibody, Immunoglobulin G3 biotin antibody, IgG3 Fab biotin antibody
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