- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Mouse IgG2c Antibody [clone RM223] (R20171)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20171-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM223 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | N/A |
| Gene ID | 404711 |
| Applications | ELISA : 0.01ug/ml-0.2ug/ml Western Blot (non-reduced Only) : 0.5-2ug/ml |
| Limitations | This recombinant Mouse IgG2c antibody is available for research use only. |
|
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The Recombinant Mouse IgG2c antibody is produced as a defined immunoglobulin reagent for use as an isotype control and assay standard. IgG2c is a subclass of mouse IgG that replaces IgG2a in certain mouse strains, such as C57BL/6, where it serves as a dominant effector antibody in immune responses. Functionally, IgG2c resembles IgG2a in its strong ability to bind Fc gamma receptors and activate complement, enabling robust cell mediated cytotoxicity and complement dependent lysis. The Recombinant Mouse IgG2c antibody captures these subclass specific structural properties while lacking antigen specificity, making it an essential tool for identifying nonspecific interactions in immunoassays.
Structurally, IgG2c shares the basic Y shaped immunoglobulin organization, with two heavy and two light chains linked by disulfide bonds. Its Fc domain interacts with activating Fc gamma receptors on natural killer cells, macrophages, and neutrophils, providing potent effector capabilities. Because IgG2c expression is strain dependent, accurate detection and use of subclass matched controls is particularly important in immunology research. The Recombinant Mouse IgG2c antibody provides this precision, offering a reliable negative control that mirrors the constant region structure of experimental IgG2c antibodies.
Applications of the Recombinant Mouse IgG2c antibody include flow cytometry, where it serves as a control for Fc receptor mediated binding and helps establish gating parameters. In immunohistochemistry, it identifies background staining in tissues infiltrated with Fc receptor bearing cells. In ELISA, the Recombinant Mouse IgG2c antibody provides a baseline for specificity, ensuring that signals originate from antigen recognition rather than nonspecific adherence. Recombinant expression guarantees lot to lot consistency, which is critical for reproducible experimental results.
This reagent is especially important for researchers working with mouse models such as C57BL/6, where IgG2c predominates and where inappropriate use of IgG2a controls could misrepresent background binding. Synonym terms such as recombinant mouse immunoglobulin G2c antibody and recombinant IgG2c isotype control antibody expand product accessibility for investigators referencing alternate nomenclature.
By delivering validated and reproducible performance, the Recombinant Mouse IgG2c antibody ensures accurate interpretation of data in immunology, oncology, and infectious disease research. NSJ Bioreagents provides the Recombinant Mouse IgG2c antibody under strict quality controls, enabling scientists to rely on its consistent behavior across platforms. With this reagent, researchers can confidently separate antigen specific activity from background noise, improving the accuracy of their immunoassays.
This recombinant Mouse IgG2c antibody reacts to the Fc region of mIgG2c. No cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2c antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Mouse IgG2c was used as the immunogen for this recombinant Mouse IgG2c antibody.
Store the recombinant Mouse IgG2c antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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