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Home >> Antibodies >> Recombinant Mouse IgG2c Antibody (Biotin Conjugate)

Recombinant Mouse IgG2c Antibody (Biotin Conjugate) [clone RM223] (R20171BTN)

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Image R20171BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.
Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.
ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM223
Purity Protein A purified from animal origin-free supernatant
UniProt N/A
Gene ID 404711
Applications ELISA : 0.01ug/ml-0.2ug/ml
Western Blot (non-reduced only) : 0.5-2ug/ml
Limitations This recombinant Mouse IgG2c antibody is available for research use only.
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Description

This recombinant Mouse IgG2c antibody reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2c antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG2c was used as the immunogen for this recombinant Mouse IgG2c antibody.

Storage

Store the recombinant Mouse IgG2c antibody at -20oC.

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   2.   View all Mouse IgG2C antibodies

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