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Home >> Antibodies >> Recombinant Mouse IgG2b Antibody

Recombinant Mouse IgG2b Antibody [clone RM108] (R20167)

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  Catalog No Formulation Size Price (USD)  
Image R20167-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM108
Purity Protein A purified from animal origin-free supernatant
UniProt P01867
Gene ID 16016
Applications ELISA : 0.005ug/ml-0.2ug/ml
Western Blot (stronger on non-reduced samples) : 0.1-0.5ug/ml
Limitations This recombinant Mouse IgG2b antibody is available for research use only.
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Description

This recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b. No cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.

Storage

Store the recombinant Mouse IgG2b antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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