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Home >> Antibodies >> Recombinant Mouse IgG2b Antibody (Biotin Conjugate)

Recombinant Mouse IgG2b Antibody (Biotin Conjugate) [clone RM108] (R20167BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20167BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
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Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/ml of the recombinant Mouse IgG2b antibody. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced form (~50 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2b antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2b. A serial dilution of recombinant Mouse IgG2b antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM108
Purity Protein A purified from animal origin-free supernatant
UniProt P01867
Gene ID 16016
Applications ELISA : 0.005ug/ml-0.2ug/ml
Western Blot (stronger on non-reduced samples) : 0.1-0.5ug/ml
Limitations This recombinant Mouse IgG2b antibody is available for research use only.
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Description

This recombinant Mouse IgG2b antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.

Storage

Store the recombinant Mouse IgG2b antibody at -20oC.

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