Mouse IgG2a-Kappa Antibody for ELISA / Anti-Mouse IgG2a-Kappa ELISA Detection Antibody [clone RM107] (R20165)

Mouse IgG2a-Kappa Antibody for WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2a kappa (20 ng per lane) using clone RM107 at 0.2 ug/mL demonstrates selective detection of the intact IgG2a kappa molecule at approximately 150 kDa under non-reducing conditions, with no detectable signal observed in the reduced sample. This binding pattern is consistent with Fab region-directed recognition requiring native immunoglobulin structure and confirms specificity for mouse IgG2a kappa. These results support the use of this Mouse IgG2a-Kappa Antibody / Anti-Mouse IgG2a-Kappa Detection Antibody for selective detection of IgG2a kappa in immunoassay applications.

Mouse IgG2a-Kappa Antibody for ELISA Fab Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that clone RM107 selectively recognizes Mouse IgG2a kappa, with strong signal observed for IgG2a kappa across tested concentrations. No cross-reactivity is detected with IgG2a lambda, IgG1, IgG3, IgM, IgA, or IgE, or with human, rat, or goat IgG. The binding profile is consistent with Fab region-directed recognition and confirms selective detection of kappa light chain-containing IgG2a. These results support the use of this Mouse IgG2a-Kappa Antibody for ELISA / Anti-Mouse IgG2a-Kappa ELISA Detection Antibody for highly specific detection in ELISA-based immunoassays.

Mouse IgG2a-Kappa Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of mouse IgG2a kappa demonstrates strong, concentration-dependent binding of clone RM107 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Mouse IgG2a kappa in ELISA-based immunoassays.