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Home >> Antibodies >> Recombinant Mouse IgG2a-Kappa Antibody (Biotin Conjugate)

Recombinant Mouse IgG2a-Kappa Antibody (Biotin Conjugate) [clone RM107] (R20165BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20165BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a-κ (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a-κ.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a-κ (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a-κ.
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2a-κ; no cross reactivity with IgG2a-lambda, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
A titer ELISA of mouse IgG2a, κ. The plate was coated with different amounts of mouse IgG2a-κ. A serial dilution of recombinant Mouse IgG2a-Kappa antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM107
Purity Protein A purified from animal origin-free supernatant
UniProt P01863
Gene ID 380793
Applications ELISA : 0.005ug/ml-0.2ug/ml
Western Blot (non-reduced only) : 0.1-0.5ug/ml
Limitations This recombinant Mouse IgG2a-Kappa antibody is available for research use only.
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Description

This recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2ak. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a-Kappa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG2a-Kappa antibody.

Storage

Store the recombinant Mouse IgG2a-Kappa antibody at -20oC.

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