Mouse IgG2a-Kappa Antibody for ELISA / Biotinylated Anti-Mouse IgG2a-Kappa ELISA Detection Antibody [clone RM107] (R20165BTN)

Mouse IgG2a-Kappa Antibody Biotin WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2a kappa (20 ng per lane) demonstrates that the parent clone RM107 antibody selectively detects intact IgG2a kappa at approximately 150 kDa under non-reducing conditions, with no detectable signal observed in the reduced sample. This pattern reflects Fab region-dependent recognition of native immunoglobulin structure and confirms specificity for mouse IgG2a kappa. These results represent the intrinsic binding characteristics of clone RM107 and are retained in the biotinylated format of the Mouse IgG2a-Kappa Antibody / Biotinylated Anti-Mouse IgG2a-Kappa Detection Antibody.

Mouse IgG2a-Kappa Antibody Biotin ELISA Fab Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that the parent clone RM107 antibody selectively recognizes Mouse IgG2a kappa, with strong signal observed for IgG2a kappa across tested concentrations. No cross-reactivity is detected with IgG2a lambda, IgG1, IgG3, IgM, IgA, or IgE, or with human, rat, or goat IgG. This binding profile reflects Fab region-directed specificity for kappa light chain-containing IgG2a and is retained in the biotinylated format of the Mouse IgG2a-Kappa Antibody for ELISA / Biotinylated Anti-Mouse IgG2a-Kappa ELISA Detection Antibody.

Mouse IgG2a-Kappa Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of mouse IgG2a kappa demonstrates strong, concentration-dependent binding of clone RM107 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM107 antibody and is representative of the characteristics retained in the biotinylated format of the Mouse IgG2a-Kappa Antibody for ELISA / Biotinylated Anti-Mouse IgG2a-Kappa ELISA Detection Antibody.