Mouse IgG2a Antibody for ELISA / Anti-Mouse IgG2a ELISA Detection Antibody [clone RM219] (R20166)

Mouse IgG2a Antibody for WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2a using clone RM219 at 0.5 ug/mL demonstrates strong detection of intact IgG2a at approximately 150 kDa under non-reducing conditions, with little to no signal observed in the reduced sample. This pattern is consistent with Fc region-directed recognition of native immunoglobulin structure and supports selective detection of mouse IgG2a independent of light chain type. These results support the use of this Mouse IgG2a Antibody / Anti-Mouse IgG2a Detection Antibody for robust detection of IgG2a in immunoassay applications.

Mouse IgG2a Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that clone RM219 selectively recognizes Mouse IgG2a, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, or IgE, or with human or rat IgG. The binding profile is consistent with Fc region-directed recognition and supports broad detection of IgG2a independent of light chain composition. These results support the use of this Mouse IgG2a Antibody for ELISA / Anti-Mouse IgG2a ELISA Detection Antibody for precise subclass-specific detection in ELISA-based immunoassays.

Mouse IgG2a Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of mouse IgG2a demonstrates strong, concentration-dependent binding of clone RM219 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Mouse IgG2a in ELISA-based immunoassays.