Mouse IgG2a Antibody for ELISA / Biotinylated Anti-Mouse IgG2a ELISA Detection Antibody [clone RM219] (R20166BTN)

Mouse IgG2a Antibody Biotin WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG2a demonstrates that the parent clone RM219 antibody selectively detects intact IgG2a at approximately 150 kDa under non-reducing conditions, with minimal signal observed in the reduced sample. This binding pattern reflects Fc region-dependent recognition of native immunoglobulin structure and supports broad detection of IgG2a regardless of light chain composition. These results represent the intrinsic binding characteristics of clone RM219 and are retained in the biotinylated format of the Mouse IgG2a Antibody / Biotinylated Anti-Mouse IgG2a Detection Antibody.

Mouse IgG2a Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that the parent clone RM219 antibody selectively recognizes Mouse IgG2a, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, or IgE, or with human or rat IgG. This binding profile reflects Fc region-directed specificity for mouse IgG2a and is retained in the biotinylated format of the Mouse IgG2a Antibody for ELISA / Biotinylated Anti-Mouse IgG2a ELISA Detection Antibody.

Mouse IgG2a Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of mouse IgG2a demonstrates strong, concentration-dependent binding of clone RM219 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM219 antibody and is representative of the characteristics retained in the biotinylated format of the Mouse IgG2a Antibody for ELISA / Biotinylated Anti-Mouse IgG2a ELISA Detection Antibody.