- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Mouse IgG1 Antibody [clone RM106] (R20164)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20164-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM106 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01868 |
| Gene ID | 16017 |
| Applications | ELISA : 0.005-0.2ug/ml Western Blot : 0.1-0.5ug/ml |
| Limitations | This recombinant Mouse IgG1 antibody is available for research use only. |
|
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The Recombinant Mouse IgG1 antibody is produced as a well defined immunoglobulin reagent designed for use as a control or reference in immunoassays. IgG1 is the most abundant subclass of immunoglobulin G in mice, representing a major component of the adaptive immune system. Its heavy chain constant region confers unique structural and functional features that distinguish it from other mouse IgG subclasses. Because of its wide prevalence in immune responses, recombinant forms of IgG1 are commonly employed to standardize assays, calibrate detection systems, and provide reliable negative controls.
Structurally, mouse IgG1 consists of two heavy chains and two light chains joined by disulfide bonds to form the classic Y shaped immunoglobulin architecture. The Fc portion of the IgG1 molecule interacts with Fc gamma receptors and complement proteins, directing effector functions such as phagocytosis and complement activation. Recombinant Mouse IgG1 antibodies are engineered to preserve this constant region while lacking antigen specific variable domains, making them valuable as isotype controls that reveal nonspecific binding of test antibodies.
The Recombinant Mouse IgG1 antibody is used widely in flow cytometry, immunohistochemistry, immunoprecipitation, and ELISA. In flow cytometry, it establishes baseline fluorescence and compensates for nonspecific interactions with Fc receptors on immune cells. In tissue staining, the reagent highlights background levels of binding that may arise from the Fc region, enabling more accurate interpretation of specific antibody signals. In ELISA, the Recombinant Mouse IgG1 antibody serves as a calibrator or background control to ensure assay specificity. Its recombinant origin guarantees lot to lot consistency, reducing variability that can occur with hybridoma derived controls.
Because IgG1 antibodies are commonly used as therapeutic and research reagents, recombinant isotype controls provide a crucial reference point. They allow researchers to confirm that observed signals are due to antigen recognition rather than structural features of the antibody itself. Synonym phrases such as recombinant mouse immunoglobulin G1 antibody and recombinant IgG1 isotype control antibody increase accessibility for users seeking equivalent products under different names.
By offering validated and reproducible detection, the Recombinant Mouse IgG1 antibody supports experimental accuracy across a range of immunological applications. NSJ Bioreagents provides the Recombinant Mouse IgG1 antibody with strict quality control, ensuring that scientists can rely on consistent performance in flow cytometry, histology, and biochemical assays. This reagent is an essential component for reliable interpretation of antibody based experiments.
This recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1. No cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mIgG2b, and may also react to goat IgG.
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Mouse IgG was used as the immunogen for this recombinant Mouse IgG1 antibody.
Store the recombinant Mouse IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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