Mouse IgG1 Antibody for ELISA / Anti-Mouse IgG1 ELISA Detection Antibody [clone RM106] (R20164)

Mouse IgG1 Antibody WB. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG1 (20 ng per lane) using clone RM106 at 0.2 ug/mL demonstrates strong detection of intact IgG1 at approximately 150 kDa under non-reducing conditions and a lower molecular weight band near 50 kDa corresponding to the gamma 1 heavy chain under reducing conditions. The stronger signal observed in the non-reduced sample is consistent with recognition of the native immunoglobulin structure, while detection of the reduced heavy chain confirms specificity for mouse IgG1. These results support the ability of this Mouse IgG1 Antibody to recognize IgG1 in both native and denatured forms.

Mouse IgG1 Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that clone RM106 selectively recognizes Mouse IgG1 / Ighg1, with strong signal observed for IgG1 across tested concentrations. The antibody shows slight cross-reactivity with IgG2b, while no reactivity is detected with IgG2a, IgG3, IgM, IgA, or IgE, or with human or rat IgG. The binding profile is consistent with Fc region-directed recognition of mouse IgG1 and supports the use of this Mouse IgG1 Antibody for ELISA / Anti-Mouse IgG1 ELISA Detection Antibody for subclass-specific detection in ELISA-based immunoassays.

Mouse IgG1 Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of mouse IgG1 demonstrates strong, concentration-dependent binding of clone RM106 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Mouse IgG1 / Ighg1 in ELISA-based immunoassays.