Mouse IgG1 Antibody for ELISA / Biotinylated Anti-Mouse IgG1 ELISA Detection Antibody [clone RM106] (R20164BTN)

Mouse IgG1 Antibody Biotin Western Blot Analysis. Western blot analysis of non-reduced (-) and reduced (+) mouse IgG1 (20 ng per lane) using clone RM106 at 0.2 ug/mL demonstrates strong detection of intact IgG1 at approximately 150 kDa under non-reducing conditions and a lower molecular weight band near 50 kDa corresponding to the gamma 1 heavy chain under reducing conditions. The stronger signal observed in the non-reduced sample is consistent with recognition of native immunoglobulin structure. These results reflect the binding characteristics of the parent clone RM106 antibody and are representative of the target recognition retained in the biotinylated format of the Mouse IgG1 Antibody for ELISA / Biotinylated Anti-Mouse IgG1 ELISA Detection Antibody.

Mouse IgG1 Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that the parent clone RM106 antibody selectively recognizes Mouse IgG1 / Ighg1, with strong signal observed for IgG1 across tested concentrations. The antibody shows slight cross-reactivity with IgG2b, while no reactivity is detected with IgG2a, IgG3, IgM, IgA, or IgE, or with human or rat IgG. This binding profile reflects Fc region-directed recognition of mouse IgG1 and is retained in the biotinylated format of the Mouse IgG1 Antibody for ELISA / Biotinylated Anti-Mouse IgG1 ELISA Detection Antibody.

Mouse IgG1 Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of mouse IgG1 demonstrates strong, concentration-dependent binding of clone RM106 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic performance of the parent clone RM106 antibody and is representative of the binding characteristics retained in the biotinylated format of the Mouse IgG1 Antibody for ELISA / Biotinylated Anti-Mouse IgG1 ELISA Detection Antibody.