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- Tel: 858.663.9055
- Email: info@nsjbio.com
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Mouse immunoglobulin G (IgG) is one of the most widely used antibody classes in research, serving as both a primary antibody species and a common component in assay development workflows. Because mouse-derived antibodies are frequently used across ELISA, western blot, and immunoassay systems, accurate and consistent detection of mouse IgG is essential for assay validation, antibody quantification, and experimental reproducibility. Detection strategies that target conserved regions of IgG are particularly valuable for ensuring uniform signal generation across diverse antibody populations and experimental conditions.
Mouse IgG antibody, also referred to as anti-mouse IgG antibody or mouse immunoglobulin G antibody, is commonly used for detection of mouse-derived antibodies in ELISA-based systems. Mouse IgG Fc Antibody for ELISA / Anti-Mouse IgG Fc Detection Antibody is specifically optimized for selective recognition of the Fc region of mouse IgG, enabling consistent detection independent of antigen-binding variability. Fc-directed detection ensures uniform recognition across mouse IgG subclasses, making it especially advantageous for assay standardization, antibody quantification, and workflows requiring reproducible and comparable results across experiments.
This recombinant goat monoclonal antibody, clone RMG06 antibody, recognizes the Fc region of mouse IgG with high specificity. Fc-region targeting allows detection of mouse antibodies regardless of antigen specificity, supporting reliable signal generation across diverse assay formats. The goat monoclonal format provides strong binding stability, reduced lot-to-lot variability, and consistent performance, which is particularly important in ELISA systems requiring low background and high reproducibility. This enables more precise measurement of antibody levels in both purified samples and complex biological matrices such as serum or cell culture supernatants.
In ELISA workflows, Fc-specific mouse IgG detection antibodies are widely used for detection of mouse primary antibodies in indirect ELISA assays, measurement of antibody titers, and assay normalization. Because the Fc region is conserved across mouse IgG subclasses including IgG1, IgG2a, IgG2b, and IgG3, detection remains consistent across different antibody populations, enabling accurate quantification and reliable comparison between samples. This is particularly important in assay development, where consistent detection across multiple antibody clones or experimental conditions is required.
Detection of mouse IgG is broadly applied in immunology research, antibody production, and assay optimization. Fc-specific detection antibodies support these applications by enabling sensitive and specific measurement of mouse IgG independent of antigen-binding variability. This antibody provides a reliable tool for ELISA-based systems requiring precise quantification, consistent signal generation, and robust performance across a wide range of experimental conditions.
This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.
The stated application concentrations are suggested starting points. Titration of the Mouse IgG Fc Antibody for ELISA / Anti-Mouse IgG Fc Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Mouse IgG was used as the immunogen for this recombinant Mouse IgG Fc antibody.
Store the recombinant Mouse IgG Fc antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Anti-mouse IgG Fc antibody, Mouse IgG Fc detection antibody, Mouse immunoglobulin G Fc antibody, Goat anti-mouse IgG Fc antibody, Fc-specific mouse IgG antibody
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