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Home >> Antibodies >> Recombinant Mouse IgG Fc Antibody

Recombinant Mouse IgG Fc Antibody [clone RMG06] (R20175)

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  Catalog No Formulation Size Price (USD)  
Image R20175-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 409
Microvalidated Recgoatmono
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fc antibody reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG06 was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fc antibody reacts to the Fc region of IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human/rat/rabbit IgG.
ELISA Titration: the plate was coated with different amounts of mouse IgG. A serial dilution of the recombinant Mouse IgG Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Goat Monoclonal
Isotype Goat IgG
Clone Name RMG06
Purity Protein G purified from animal origin-free supernatant
UniProt N/A
Gene ID N/A
Applications ELISA : 0.05ug/ml-1ug/ml
Limitations This recombinant Mouse IgG Fc antibody is available for research use only.
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Description

This recombinant Mouse IgG Fc antibody reacts to the Fc region of mIgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE or human/rat/rabbit IgG..

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG Fc antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG Fc antibody.

Storage

Store the recombinant Mouse IgG Fc antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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