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Home >> Antibodies >> Recombinant Mouse IgG Fab Antibody

Recombinant Mouse IgG Fab Antibody [clone RMG05] (R20174)

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  Catalog No Formulation Size Price (USD)  
Image R20174-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 409
Microvalidated Recgoatmono
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fab antibody reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG05 was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fab antibody reacts to the Fab region of IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human/rat/rabbit IgG.
ELISA Titration: the plate was coated with different amounts of mouse IgG. A serial dilution of recombinant Mouse IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Goat Monoclonal
Isotype Goat IgG
Clone Name RMG05
Purity Protein G purified from animal origin-free supernatant
Gene ID N/A
Applications ELISA : 0.05ug/ml-1ug/ml
Limitations This recombinant Mouse IgG Fab antibody is available for research use only.
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Description

This recombinant Mouse IgG Fab antibody reacts to the Fab region of mIgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE or human/rat/rabbit IgG..

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG Fab antibody.

Storage

Store the recombinant Mouse IgG Fab antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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