Mouse IgG Antibody for ELISA / Fc-Specific Anti-Mouse IgG Detection Antibody [clone RM104] (R20163)

Mouse IgG Antibody for WB Subclass Reactivity Analysis. Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins demonstrates that clone RM104 selectively recognizes mouse IgG subclasses, with strong signal observed for nonreduced IgG1, IgG2a, IgG2b, and IgG3, consistent with Fc-region-dependent binding. Signal is markedly reduced under reducing conditions, reflecting disruption of Fc structural integrity. No cross-reactivity is detected with IgM, IgA, or IgE. These results support Fc-specific recognition and highlight the utility of this Mouse IgG Antibody / Fc-Specific Anti-Mouse IgG Detection Antibody for selective IgG detection.

Mouse IgG Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that clone RM104 selectively recognizes mouse IgG / Ighg through Fc-region binding, with strong signal observed for IgG1, IgG2a, IgG2b, and IgG3 across tested concentrations. No cross-reactivity is detected with IgM, IgA, or IgE, and no reactivity is observed with human or rat IgG. This binding profile supports Fc-specific subclass-independent detection and highlights the utility of this Mouse IgG Antibody for ELISA / Fc-Specific Anti-Mouse IgG Detection Antibody for total IgG measurement in ELISA-based immunoassays.

Mouse IgG Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of mouse IgG demonstrates strong, concentration-dependent binding of clone RM104 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of mouse IgG / Ighg in ELISA-based immunoassays.