Mouse IgG Antibody for ELISA / Biotinylated Fc-Specific Anti-Mouse IgG Detection Antibody [clone RM104] (R20163BTN)

Mouse IgG Antibody Biotin Western Blot Subclass Reactivity Analysis. Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins demonstrates that the parent clone RM104 antibody selectively recognizes mouse IgG subclasses, with strong signal observed for nonreduced IgG1, IgG2a, IgG2b, and IgG3, consistent with Fc-region-dependent binding. Signal is reduced under reducing conditions due to disruption of Fc conformation. No cross-reactivity is detected with IgM, IgA, or IgE. This binding profile reflects Fc-specific recognition and is representative of the characteristics retained in the biotinylated format of the Mouse IgG Antibody / Biotinylated Fc-Specific Anti-Mouse IgG Detection Antibody.

Mouse IgG Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of mouse immunoglobulins demonstrates that the parent clone RM104 antibody selectively recognizes mouse IgG / Ighg through Fc-region binding, with strong signal observed for IgG1, IgG2a, IgG2b, and IgG3 across tested concentrations. No cross-reactivity is detected with IgM, IgA, or IgE, and no reactivity is observed with human or rat IgG. This binding profile reflects Fc-specific recognition and is representative of the performance retained in the biotinylated format of the Mouse IgG Antibody for ELISA / Biotinylated Fc-Specific Anti-Mouse IgG Detection Antibody.

Mouse IgG Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of mouse IgG demonstrates strong, concentration-dependent binding of clone RM104 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM104 antibody and is representative of the characteristics retained in the biotinylated format of the Mouse IgG Antibody for ELISA / Biotinylated Fc-Specific Anti-Mouse IgG Detection Antibody.