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Home >> Antibodies >> Recombinant MLH1 Antibody / MutL Homolog 1

Recombinant MLH1 Antibody / MutL Homolog 1 [clone MLH1/6284R] (V9349)

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  Catalog No Formulation Size Price (USD)  
Image V9349-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9349-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9349SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human colon carcinoma tissue with recombinant MLH1 antibody (clone MLH1/6284R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma tissue with recombinant MLH1 antibody (clone MLH1/6284R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (clone MLH1/6284R) at 2ug/ml in PBS, 30 min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (MLH1/6284R) at 2ug/ml.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name MLH1/6284R
Purity Protein A/G affinity
UniProt P40692
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant MLH1 antibody is available for research use only.
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Description

This MAb recognizes a protein of 83kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

Application Notes

Optimal dilution of the recombinant MLH1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human MLH1 protein was used as the immunogen for the recombinant MLH1 antibody.

Storage

Aliquot the recombinant MLH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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