MLANA Antibody / Melanocyte Lineage Marker Antibody [clone rMLANA/9404] (V5603)

MLANA Antibody human COLO-38 cell WB analysis. Western blot analysis of Melan A (MLANA) in human COLO-38 cell lysate using MLANA Antibody / Melanocyte Lineage Marker Antibody clone rMLANA/9404 shows a band at approximately 20 kDa. A band is detected at approximately 20 kDa, consistent with the predicted molecular weight of Melan A (MLANA), with a possible doublet representing isoform variation or post-translational processing. The observed signal aligns with the known expression of this melanocyte lineage marker in melanoma-derived cell lines.

MLANA Antibody human skin IHC staining. Immunohistochemistry analysis of Melan A (MLANA) in FFPE human skin using MLANA Antibody / Melanocyte Lineage Marker Antibody clone rMLANA/9404 demonstrates cytoplasmic HRP-DAB brown staining in melanocytes localized along the basal layer of the epidermis, consistent with melanocyte lineage distribution, while surrounding keratinocytes and dermal cells remain largely negative. The staining pattern highlights discrete melanocyte populations with clear localization and minimal background signal. The inset shows a negative control with PBS in place of the primary antibody, confirming staining specificity. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer for 20 min prior to staining.

SDS-PAGE analysis of purified, BSA-free recombinant MLANA antibody (clone rMLANA/9404) as confirmation of integrity and purity.