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Home >> Antibodies >> Recombinant Melan-A Antibody

Recombinant Melan-A Antibody [clone rMLANA/788] (V3717)

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  Catalog No Formulation Size Price (USD)  
Image V3717-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3717-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3717SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3717IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone rMLANA/788). Expected molecular weight ~20 kDa with possible doublet.
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Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone rMLANA/788). Expected molecular weight ~20 kDa with possible doublet.
SDS-PAGE analysis of purified, BSA-free recombinant Melan-A antibody (clone rMLANA/788) as confirmation of integrity and purity.
IHC testing of FFPE human melanoma with recombinant Melan-A antibody (clone rMLANA/788). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rMLANA/788
Purity Protein G affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This recombinant Melan-A antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Application Notes

Optimal dilution of the recombinant Melan-A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human MLANA protein was used as the immunogen for the recombinant Melan-A antibody.

Storage

Store the recombinant Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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