MCM7 Antibody Recombinant Mouse MAb rMCM7/1468 / Minichromosome Maintenance Protein 7 Antibody [clone rMCM7/1468] (V7947)

MCM7 Antibody Recombinant Mouse MAb rMCM7/1468 - western blot analysis. Western blot testing of human cell lysates using MCM7 Antibody Recombinant Mouse MAb rMCM7/1468. Lane 1: MCF7 cell lysate, Lane 2: HEK293 cell lysate, Lane 3: A549 cell lysate, Lane 4: SKBR3 cell lysate, Lane 5: HeP2 cell lysate. A band is detected at approximately 80-90 kDa, consistent with the predicted molecular weight of Minichromosome maintenance protein 7 / MCM7. Additional lower bands may represent lower molecular weight MCM7 forms or partial proteolytic degradation products, as MCM7 has been reported in the literature as an approximately 82-85 kDa doublet or broad band on immunoblot analysis.

IHC testing of FFPE human tonsil with recombinant MCM7 antibody (clone rMCM7/1468). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

MCM7 Antibody Recombinant Mouse MAb rMCM7/1468 - protein microarray specificity validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins was performed using MCM7 Antibody Recombinant Mouse MAb rMCM7/1468. The strongest binding signal corresponds to MCM7, which ranks first on the array, demonstrating high specificity of the rMCM7/1468 monoclonal antibody for its intended target. Z- and S-scores quantify antibody binding strength and target specificity. The Z-score represents the signal intensity generated when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a protein on the array and is expressed as standard deviations above the mean signal of the array. When proteins are ordered by descending Z-score, the S-score represents the difference between adjacent Z-scores and therefore reflects the relative specificity of the antibody for its target protein.

SDS-PAGE analysis of purified, BSA-free recombinant MCM7 antibody (clone rMCM7/1468) as confirmation of integrity and purity.

Western blot testing of human A431 cell lysate with recombinant MCM7 antibody (clone rMCM7/1468). Expected molecular weight: 80-90 kDa.