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Home >> Antibodies >> Recombinant MBP Antibody / Myelin Basic Protein

Recombinant MBP Antibody / Myelin Basic Protein [clone rMBP/4288] (V8437)

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  Catalog No Formulation Size Price (USD)  
Image V8437-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8437-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8437SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human cerebellum with recombinant MBP antibody (clone rMBP/4288). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human cerebellum with recombinant MBP antibody (clone rMBP/4288). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebellum with recombinant MBP antibody (clone rMBP/4288). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain lysate with recombinant MBP antibody (clone rMBP/4288). Isoforms may be visualized from 20~37 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant MBP antibody (clone rMBP/4288) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rMBP/4288
Purity Protein G affinity chromatography
UniProt P02686
Localization Cell surface, cytoplasm
Applications Western blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant MBP antibody is available for research use only.
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Description

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.

Application Notes

Optimal dilution of the recombinant MBP antibody should be determined by the researcher.

Immunogen

A portion of amino acids 150-250 from the human protein was used as the immunogen for the recombinant MBP antibody.

Storage

Store the recombinant MBP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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