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Home >> Antibodies >> Recombinant MART-1 Antibody / Rabbit Monoclonal

Recombinant MART-1 Antibody / Rabbit Monoclonal [clone MLANA/1409R] (V7267)

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  Catalog No Formulation Size Price (USD)  
Image V7267-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7267-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7267SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7267IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone MLANA/1409R). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone MLANA/1409R). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone MLANA/1409R). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant MART-1 antibody (clone MLANA/1409R) as confirmation of integrity and purity.
Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone MLANA/1761R). Expected molecular weight ~20 kDa with possible doublet.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MLANA/1409R
Purity Protein A affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This recombinant MART-1 antibody is available for research use only.
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Description

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Application Notes

The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human protein was used as the immunogen for this recombinant MART-1 antibody.

Storage

Store the recombinant MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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