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Home >> Antibodies >> Recombinant MART-1 Antibody / Melan-A

Recombinant MART-1 Antibody / Melan-A [clone MLANA/4385R] (V8729)

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  Catalog No Formulation Size Price (USD)  
Image V8729-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8729-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8729SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human skin with recombinant MART-1 antibody (clone MLANA/4385R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skin with recombinant MART-1 antibody (clone MLANA/4385R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human COLO-38 cell lysate with recombinant MART-1 antibody (clone MLANA/4385R). Expected molecular weight ~20 kDa with possible doublet.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name MLANA/4385R
Purity Protein A affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Western blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant MART-1 antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Application Notes

Optimal dilution of the recombinant MART-1 antibody should be determined by the researcher.

Immunogen

A recombinant fragment corresponding to the N-terminus of human Melan A was used as the immunogen for the recombinant MART-1 antibody.

Storage

Store the recombinant MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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