Mammaglobin Antibody for IF / SCGB2A2 Antibody [clone MGB/2682R] (V7436)

Mammaglobin Antibody immunohistochemistry of human breast carcinoma. FFPE human breast carcinoma tissue was stained with recombinant Mammaglobin antibody (clone MGB/2682R). HRP-DAB brown chromogenic signal highlights cytoplasmic staining in tumor epithelial cells forming glandular structures, consistent with expression of Mammaglobin A / SCGB2A2 in breast carcinoma cells, while surrounding stromal cells show little to no staining. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM citrate buffer (pH6) for 10-20 minutes followed by cooling at room temperature prior to staining.

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa, MCF-7, SK-Br3 and T47D cell lysate with recombinant Mammaglobin antibody (clone MGB/2682R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone MGB/2682R) as confirmation of integrity and purity.

Mammaglobin Antibody for IF immunofluorescence staining of human MCF7 cells. Permeabilized human MCF7 breast carcinoma cells were stained with Mammaglobin Antibody for IF (clone MGB/2682R). Green fluorescence highlights Mammaglobin A / SCGB2A2 expression within the cytoplasm of tumor epithelial cells, consistent with the localization of this secreted breast-associated protein. Nuclei are counterstained with Reddot nuclear stain (red), allowing visualization of cell morphology and confirming cytoplasmic distribution of the Mammaglobin signal in breast cancer cells.