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Home >> Antibodies >> Recombinant Mammaglobin A Antibody / SCGB2A2

Recombinant Mammaglobin A Antibody / SCGB2A2 [clone MMGA-1R] (V7309)

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  Catalog No Formulation Size Price (USD)  
Image V7309-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7309-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7309SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7309IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human MCF-7 cell lysate with recombinant Mammaglobin A antibody (clone MMGA-1R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.
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Western blot testing of human MCF-7 cell lysate with recombinant Mammaglobin A antibody (clone MMGA-1R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.
IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin A antibody (clone MMGA-1R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling prior to testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MMGA-1R
Purity Protein A affinity chromatography
UniProt Q13296
Localization Cytoplasmic, cell surface, nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant Mammaglobin A antibody is available for research use only.
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Description

SCGB2A2 is a member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seem to be involved in cell signalling, immune response, and chemotaxis, and may also serve as transporters for steroid hormones in humans. SCGB2A2 expression is highly specific of mammary tissue, and is increasingly used for identification and detection of disseminated breast cancer cells.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mammaglobin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length recombinant human protein was used as the immunogen for the recombinant Mammaglobin A antibody.

Storage

Store the recombinant Mammaglobin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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