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Home >> Antibodies >> Recombinant LGALS3 Antibody rLGALS3/7286 / Galectin-3 Recombinant Mouse MAb

Recombinant LGALS3 Antibody rLGALS3/7286 / Galectin-3 Recombinant Mouse MAb [clone rLGALS3/7286] (V4350)

  Catalog No Formulation Size Price (USD)  
Image V4350-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4350-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4350SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
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Western blot testing of human MCF7 cell lysate with recombinant LGALS3 antibody (clone rLGALS3/7286). Predicted molecular weight ~26 kDa.
IHC staining of FFPE human colon tissue with Recombinant LGALS3 Antibody rLGALS3/7286. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant LGALS3 antibody (clone rLGALS3/7286) as confirmation of integrity and purity.
Recombinant LGALS3 Antibody by WB in human tissues and cell lines. Western blot analysis of human heart, lung, and skin tissue lysates, along with A431 and PANC-28 cell lysates, using Recombinant LGALS3 Antibody rLGALS3/7286. A band is detected at approximately 29 kDa, consistent with the expected molecular weight of galectin-3.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rLGALS3/7286
Purity Protein A/G affinity
UniProt P17931
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This Recombinant LGALS3 Antibody rLGALS3/7286 is available for research use only.
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Description

Galectin-3 is a member of the beta-galactosidase-binding lectin family. It is associated with cell growth, adhesion, inflammation, mRNA processing, and apoptosis. Aberrant expression of Galectin-3 is related to malignant transformation and metastasis in carcinomas of the breast, colon and thyroid. Galectin-3 reactivity can be seen in the nucleus of neutrophils, vascular endothelium, carcinomas of the colon, breast, and thyroid. Galectin-3 may be useful in the differentiation of benign and malignant thyroid neoplasms. Galectin-3 may also be useful in the identification of certain liver disorders.

Explore our Galectin-3 Antibody LGALS3/6583 for additional research tools targeting LGALS3, with Western blot, immunohistochemistry, and HuProt protein microarray validation.

Application Notes

Optimal dilution of the Recombinant LGALS3 Antibody rLGALS3/7286 should be determined by the researcher.

Immunogen

A recombinant fragment from the human protein was used as the immunogen for the recombinant LGALS3 antibody.

Storage

Aliquot the recombinant LGALS3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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