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Home >> Antibodies >> Recombinant LC3B Antibody / N-Terminal

Recombinant LC3B Antibody / N-Terminal [clone RM293] (R20312)

  Catalog No Formulation Size Price (USD)  
Image R20312-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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ICC staining of human HeLa cells untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody (red) at 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
Western blot testing of lysate from HeLa cells, untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody at 1:200 dilution. Predicted molecular weight ~15 kDa.
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant LC3B antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM293
Purity Protein A purified from animal origin-free supernatant
UniProt Q9GZQ8
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Immunocytochemistry : 1:100-1:200
Western Blot : 1:100-1:200
Limitations This recombinant LC3B antibody is available for research use only.
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Description

This antibody reacts to human LC3B (Autophagy-related protein LC3 B; Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.

Storage

Store the recombinant LC3B antibody at -20oC.

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